The mechanism of in vitro clot lysis induced by vascular plasminogen activator
نویسندگان
چکیده
منابع مشابه
The mechanism of in vitro clot lysis induced by vascular plasminogen activator.
The contribution of vascular plasminogen activator (v-PA) to the lysis of whole blood and plasma clots was investigated. v-PA released into the circulation after infusion of deamino-D-arginine vasopressin (DDAVP) was shown to bind quantitatively to plasma clots. Its apparent molecular weight, determined by the SDS-PAGE fibrin-agarose underlay method, was approximately 68,000 daltons, and its ac...
متن کاملDual frequency ultrasound-enhanced tissue plasminogen activator thrombolysis in an in vitro human clot model
Introduction: Stroke causes death and disability in patients throughout the world. At present, the only FDA- approved drug for ischemic stroke is recombinant tissue plasminogen activator (rt- PA). Unfortunately, rtPA can cause intracerebral hemorrhage and must use within limited time window (within 3-4.5 hour after onset of stroke). Ultrasound with rtPA loaded liposomes (rtPA_L...
متن کاملMechanism of protein C-dependent clot lysis: role of plasminogen activator inhibitor.
The mechanism by which activated protein C stimulates fibrinolysis was studied in a simple radiolabeled clot lysis assay system containing purified tissue-type plasminogen activator, bovine endothelial plasminogen activator inhibitor (PAI), plasminogen, 125I-fibrinogen and thrombin. Fibrinolysis was greatly enhanced by the addition of purified bovine activated protein C; however, in the absence...
متن کاملInhibition of Clot Lysis and Decreased Binding of Tissue-Type Plasminogen Activator as a Consequence of Clot Retraction
Tissue-type plasminogen activator (t-PA) is less active in vivo and in vitro against clots that are enriched in platelets, even at therapeutic concentrations. The release of radioactivity from ‘El-fibrin-labeled clots was decreased by 47% 6 hours after the addition of t-PA 400 U/mL when formed in plateletrich versus platelet-poor plasma. This difference was not due to the release of plasminogen...
متن کاملSpeed of Intracranial Clot Lysis With Intravenous Tissue Plasminogen Activator Therapy
Background—Arterial recanalization precedes clinical improvement or may lead to hemorrhage or reperfusion injury. Speed of clot lysis was not previously measured in human stroke. Methods and Results—Transcranial Doppler (TCD) and the National Institutes of Health Stroke Scale (NIHSS) were used to monitor consecutive patients receiving intravenous tissue plasminogen activator (tPA), before tPA b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Blood
سال: 1984
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v63.6.1331.1331